Coding

Part:BBa_K942001:Design

Designed by: Luis Mario Leal Garza   Group: iGEM12_Tec-Monterrey   (2012-09-26)


scFv anti-His


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 70
    Illegal BglII site found at 236
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon-optimized to be expressed in yeast. It is composed by two domains fused by a (GGGS)4 linker.


Source

As a fusion protein, the DNA of this scFv was synthetized artificially, its parts comes from a murine antibody.

References